The effect of ionization and tautomerization. Shulman (1966) Luminescence of pyrimidines, purines, nucleosides and nucleotides at 77 ÃÂðK. Absorbance measurements of human genomic DNA and synthetic oligonucleotides can be done quickly and directly with spectrophotometers that are readily calibrated. D., Editor (1975) Handbook of Biochemistry and Molecular Biology, 3rd Edition, Nucleic Acids, Volume I, pp. The absorbance values for the human genomic DNA sample used in this study gave consistent mass concentration values for the native samples and the sample denatured by NaOH (Table 4). Lindsey (1998) PhotochemCAD: A computer-aided design and research tool in photochemistry. 9, 10 The results from these studies provide important qualitative information regarding the melanin absorption properties in its native environment, but the empirical nature of data. A Refined Program with Accompanying Spectral Databases for Photochemical Calculations, Photochem. The absorption spectrum of melanin has been previously investigated in vivo using diffuse reflectance spectroscopy by using empirical methods for data analysis. (1979) Polarized fluorescence and estimated lifetimes of the DNA bases at room temperature. In the case of nucleic acids (DNA and RNA), the UV absorbance from 200 to 300 nm is due exclusively to transitions of the planar purine and pyrimidine bases. For fluorescence studies of the nucleic acid bases and related heterocyclic compounds, see (Longworth, 1966).Ĭallis, P. Absorbance spectral scans of purified dsDNA, protein, or a mixture. The value of 7,900cm -1/M at 263.8nm (Fasman, 1975).ĭue to the expected low fluorescence yield, estimated to be 0.000102 (Callis, 1979), a fluorescence spectrum was not collected. Nucleic acids are often characterized and quantified using their absorption spectra, as measured by spectrophotometry. Double-stranded DNA spectral scanning measurements were made using an Agilent. To use the viewer for absorbance dyes (colorimetric format labels), click the left drop-down menu under 'Current mode. The Spectrum Viewer is set to fluorescent mode by default. These measurements were scaled to make the molar extinction coefficient match AAT Bioquests interactive Spectrum Viewer is a powerful tool for analysis and comparison of the spectra of common compounds used in absorbance and fluorescence formats. ![]() The absorption values were collected using a spectral bandwidth of 1.0 nm, a signal averaging time of 0.133 sec, a data interval of 0.25 nm, and a scan rate of 112.5 nm/min. Fuh in the summer of 1995 using a Cary 3. This optical absorption measurement of Thymine were made by
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